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rabbit polyclonal antitranscription factor iib tfiib antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit polyclonal antitranscription factor iib tfiib antibody
    Rabbit Polyclonal Antitranscription Factor Iib Tfiib Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antitranscription factor iib tfiib antibody/product/Santa Cruz Biotechnology
    Average 93 stars, based on 191 article reviews
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    93/100 stars

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    Recruitment of the transcription machinery to DNA centers. Confocal micrographs of a control (non-infected) HeLa cell and a cell infected with Ad5 wt (1000 VP/cell) imaged at 21 hpi. (A) Nascent transcripts were labeled using BrUTP in permeabilized cells. Triple staining was performed using anti-BrdU (green), anti-72 kD (blue) and anti-TBP (red) antibodies. (B) Control and infected cells stained with <t>anti-TFIIB</t> and anti-72 kD and (C) anti-TFIIF and anti-72 kD antibodies.
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    Journal: The EMBO Journal

    Article Title: BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses

    doi: 10.15252/embj.2018100293

    Figure Lengend Snippet:

    Article Snippet: Rabbit anti‐TFIIB , Santa Cruz , Cat# sc‐271736, RRID:AB_10709889.

    Techniques: Recombinant, Positive Control, Microarray, RNA Sequencing, ChIP-sequencing

    Recruitment of the transcription machinery to DNA centers. Confocal micrographs of a control (non-infected) HeLa cell and a cell infected with Ad5 wt (1000 VP/cell) imaged at 21 hpi. (A) Nascent transcripts were labeled using BrUTP in permeabilized cells. Triple staining was performed using anti-BrdU (green), anti-72 kD (blue) and anti-TBP (red) antibodies. (B) Control and infected cells stained with anti-TFIIB and anti-72 kD and (C) anti-TFIIF and anti-72 kD antibodies.

    Journal: Experimental cell research

    Article Title: Nuclear Actin and Myosins in Adenovirus Infection

    doi: 10.1016/j.yexcr.2015.07.025

    Figure Lengend Snippet: Recruitment of the transcription machinery to DNA centers. Confocal micrographs of a control (non-infected) HeLa cell and a cell infected with Ad5 wt (1000 VP/cell) imaged at 21 hpi. (A) Nascent transcripts were labeled using BrUTP in permeabilized cells. Triple staining was performed using anti-BrdU (green), anti-72 kD (blue) and anti-TBP (red) antibodies. (B) Control and infected cells stained with anti-TFIIB and anti-72 kD and (C) anti-TFIIF and anti-72 kD antibodies.

    Article Snippet: The following primary antibodies were used: the monoclonal antibody B610 against the viral 72-kDa (72K) DNA binding protein to identify viral replication centers (kindly provided by Dr. Arnold J. Levine, Institute for Advanced Study) [ 52 ], the monoclonal antibody to β-actin produced in mouse (clone AC-15, ascites fluid, Sigma Aldrich), the mouse monoclonal antibody 2G2 against actin [ 53 ] (kindly provided by Dr. Brigitte Jockusch, Technical University of Braunschweig, Germany), the mouse monoclonal anti-BrdU antibody (clone BMC 9318, Roche) used to detect nascent RNA following Br-UTP incorporation, the rabbit polyclonal antibody specific for TBP (Santa Cruz Biochemical), the mouse monoclonal H14 antibody to phosphorylated serine 5 of the C-terminal domain of pol II (Covance) or the polyclonal pan-Pol II antibody (n-20, Santa Cruz), the rabbit polyclonal anti-TFIIB antibody (Santa Cruz), the rabbit polyclonal anti-TFIIF antibody (Santa Cruz), rhodamine phalloidin (Cytoskeleton Inc.), polyclonal myosin V antibody (kindly provided by Dr. Paul C. Bridgman, Washington University), polyclonal myosin VI antibody (KA-15, Sigma Aldrich) and the rabbit anti-nuclear myosin I (NMI) antibody developed in our laboratory.

    Techniques: Control, Infection, Labeling, Staining